The Study and Characterization of Primary Squamous Cell Carcinoma of the Head and Neck and Their Malignant Potential

Konference: 2015 XI. Dny diagnostické, prediktivní a experimentální onkologie

Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie

Téma: Postery

Číslo abstraktu: p07

Autoři: Ing. Simona Dostálová; Mgr. Martina Raudenská, Ph.D.; Mgr. Markéta Svobodová; Bc. Michaela Fojtů; Ing. Hana Polanská; RNDr. Jan Balvan; Marek Feith; MUDr. Jaromír Gumulec, Ph.D.; MUDr. Hana Binková, Ph.D.; MUDr. Zuzana Horáková; prof. MUDr. Rom Kostřica, CSc.; doc., RNDr. Michal Masařík, Ph.D.; Mgr. Zbyněk Heger; Ana Maria Jimenez, MSc.; Mgr. Ondřej Zítka; doc.RNDr. Vojtěch Adam, Ph.D.; doc.Ing. René Kizek, Ph.D.

Introduction

Squamous cell carcinomas (HNSCC) form 90% of all head and neck cancers. Even with advanced therapy and operative skills, the 5-year survival rate is no more than 50% [1]. HNSCC are not only caused by tobacco and alcohol abuse, there is also a huge involvement of HPV infections [2]. New potentially clinically useful biomarkers are being studied, such as metallothioneins (MT) [3] or miRNAs [4].

Materials/methods

Samples from almost 300 HNSCC positive and healthy patients were tested - biopsy of tumour tissue, adjacent healthy tissue or leukocytes were used in this study and compared with non-cancer individuals. Standard PCR or qRTPCR methods were employed to detect HPV or expression of certain genes.

Results and conclusions

54% of studied HNSCC patients were HPV-positive, with 21% of HPV 16 subtype, 13% of HPV 18 subtype and 7% of HPV 16 and 18 types co-infection. The expression of commonly studied HNSCC markers MT2, MMP9, FLT1, VGFA and POU5F was significantly inhibited in HPV-positive HNSCC patients, compared with HPV-negative patients. The HPV-positive patients also showed better disease-specific survival. Moreover, expression of other genes was also studied. MT2A, BAX, EGF and JUN showed increased expression in the healthy tissues surrounding the tumour tissue and a significant shift of BAX/BCL2 ratio was observed. The adjacent healthy tissue was also shown to release several tumoursupporting factors, increasing cancerogenesis. Also, an increase in the expression of the protein metallothionein was observed, in tumour-adjacent tissue and more so in the tumours, evidencing it as a potential HNSCC biomarker. Based on this information, microfluidic label-free bead-based, portable metallothionein immunosensor with electrochemical detection was designed with LOD of 12.5 ng/mL.

The authors gratefully acknowledge financial support from the SPINCANCER NT/14337-3/2013.

  1. Torre, L. A. et al. Global Cancer Statistics, 2012. CA-Cancer J. Clin. 65, 87-108, (2015).
  2. Gillison, M. L. et al. Epidemiology of Human Papillomavirus-Positive Head and Neck Squamous Cell Carcinoma. Journal of clinical oncology : official journal of the American Society of Clinical Oncology 33, 3235-3242, (2015).
  3. Dutsch-Wicherek, M. et al. Metallothionein stroma reaction in tumor adjacent healthy tissue in head and neck squamous cell carcinoma and breast adenocarcinoma. Neuroendocrinol. Lett. 26, 567-574, (2005).
  4. Hauser, B. et al. Functions of MiRNA-128 on the Regulation of Head and Neck Squamous Cell Carcinoma Growth and Apoptosis. PLoS One 10, 1-10, (2015).

Datum přednesení příspěvku: 2. 12. 2015