Konference: 2006 31st Congress ESMO - účast ČR
Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie
Téma: Molecular markers
Číslo abstraktu: 874P
Autoři: Doc. MUDr. Luboš Holubec (jr.), Ph.D., MBA; RNDr. Martin Pešta, Ph.D.; prof. MUDr. Jindřich Fínek, Ph.D.; Prof. MUDr. Ondřej Topolčan, CSc.; Karel Ruprt; Prof. MUDr. Vladislav Třeška, DrSc.
Objectives: The aim of our study was to correlate levels of
MMP-2, MMP-7, TIMP-1 and TIMP-2 mRNA in colorectal carcinoma tissue
samples with the clinicopathological status of the disease.
Methods: Colorectal carcinoma tissue samples were obtained from 38 patients who underwent resection of colorectal carcinoma. The expression levels of mRNA MMP-2, MMP-7, TIMP-1, TIMP-2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene were quantified in these tissue samples using the method of reverse transcription real-time PCR.
Results: It was found that the level of mRNA expression of MMP-2, TIMP-2, MMP-7 and TIMP-1 was significantly higher in tumor tissue samples than in the normal colorectal tissue (p<0.0005, resp.<0.0007 and resp.<0.0004). The level of mRNA expression of MMP-2, MMP-7, TIMP-2 and TIMP-1 did not correlate with the stage of the disease, with localization of the tumor , with metastatic spread nor with disease free survival (DFI). We recorded a reciprocal negative statistically significant correlation (r=-0.85; p<0.0001) between the levels of mRNA MMP-7 and mRNA TIMP-2. Correlations between the values of mRNA MMP-7 vs. TIMP-1, MMP-2 vs. TIMP-2, MMP-2 vs. TIMP-1 and MMP-2 vs. MMP-7 were not statistically significant.
Conclusions: We found that there were statistically significant differences in the levels of MMP-2, MMP-7, TIMP-1, TIMP-2 mRNA between normal colorectal tissue and tumor tissue. We found a reciprocal negative statistically significant correlation between levels of mRNA MMP-7 and TIMP-2. On the basis of these results the clinical use of this approach to the determination of a prognosis is ambiguous.
This study was supported by the grant VZ MSM 0021620819 and IGA NR 7894-3.
Methods: Colorectal carcinoma tissue samples were obtained from 38 patients who underwent resection of colorectal carcinoma. The expression levels of mRNA MMP-2, MMP-7, TIMP-1, TIMP-2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene were quantified in these tissue samples using the method of reverse transcription real-time PCR.
Results: It was found that the level of mRNA expression of MMP-2, TIMP-2, MMP-7 and TIMP-1 was significantly higher in tumor tissue samples than in the normal colorectal tissue (p<0.0005, resp.<0.0007 and resp.<0.0004). The level of mRNA expression of MMP-2, MMP-7, TIMP-2 and TIMP-1 did not correlate with the stage of the disease, with localization of the tumor , with metastatic spread nor with disease free survival (DFI). We recorded a reciprocal negative statistically significant correlation (r=-0.85; p<0.0001) between the levels of mRNA MMP-7 and mRNA TIMP-2. Correlations between the values of mRNA MMP-7 vs. TIMP-1, MMP-2 vs. TIMP-2, MMP-2 vs. TIMP-1 and MMP-2 vs. MMP-7 were not statistically significant.
Conclusions: We found that there were statistically significant differences in the levels of MMP-2, MMP-7, TIMP-1, TIMP-2 mRNA between normal colorectal tissue and tumor tissue. We found a reciprocal negative statistically significant correlation between levels of mRNA MMP-7 and TIMP-2. On the basis of these results the clinical use of this approach to the determination of a prognosis is ambiguous.
This study was supported by the grant VZ MSM 0021620819 and IGA NR 7894-3.
Datum přednesení příspěvku: 29. 6. 2006
