Konference: 2008 33st Congress ESMO - účast ČR
Kategorie: Genitourinární nádory
Téma: Genitourinary tumors
Číslo abstraktu: 608P
Autoři: doc. MUDr. Otakar Kopecký, CSc.; MUDr. Šárka Lukešová, Ph.D.; Mgr. Vladimíra Vroblová, Ph.D.; Doc.RNDr. Ctirad Andrýs, Ph.D.; MUDr. Dagmar Hlávková, Ph.D.; MUDr. Petr Morávek, CSc.
Goal: To confirm whether the removal of the primary renal
cell carcinoma (RCC) will be accompanied by changes in levels of
angiogenic factors in serum.
Patients: RCC was diagnosed in 32 patients (the average age was 65.9 ± 10,8 years). In all the patients the tumour was removed. The diagnosis of clear cell RCC was confirmed histologically. The patients were divided into three groups according to the disease stages. (AJCC staging, 6th Edition, 2002). The first group included 15 patients with stages I and II, the second group consisted of eight patients with stage III and the third group nine patients with stage IV. The control group consisted of 14 healthy volunteers with an average age of 53.7±7,9.
Method: The levels of angiogenic factors in the serum samples taken before surgery, on day seven and week eight after surgery were determined by means of a protein array method by RayBiotech Company (USA), RayBio Human Angiogenesis Antibody Array I. The evaluation was carried out by ARES ARay (Baria, Czech Rep.). The resulting concentration of proteins was expressed as a relative value in comparison to controls.
Results: We found a significant pre-operative increase in IL-8 level in patients with stage III RCC (p=0.040) and stage IV RCC (p=0.017) compared to the control group. There was a significant post-operative decrease in IL-8 levels in all the groups of patients as early as day seven after surgery. Pre-operative serum levels of PDGF in patients with stages I and II RCC (p=0.017) and stage III RCC (p=0.029) were significantly increased compared to control group. On the contrary, in patients with stage IV RCC, it was lower compared to control group (p=0.032) and patients with stages I and II RCC (p<0.001) and stage III RCC (p=0.005). In patients with stage IV RCC the increase in PDGF reached statistical significance (p=0.003) on week eight.
Conclusion: The decrease in IL-8 in serum as early as day seven after surgery shows its significant production by the primary RCC cells. PDGF levels in serum did not change after the removal of primary RCC or they were gradually increasing in stage IV RCC, which proves other regulatory pathway than in IL-8. Supported by the grant from the IGA of the Czech Republic Ministry of Health: NR/8914-4.
Patients: RCC was diagnosed in 32 patients (the average age was 65.9 ± 10,8 years). In all the patients the tumour was removed. The diagnosis of clear cell RCC was confirmed histologically. The patients were divided into three groups according to the disease stages. (AJCC staging, 6th Edition, 2002). The first group included 15 patients with stages I and II, the second group consisted of eight patients with stage III and the third group nine patients with stage IV. The control group consisted of 14 healthy volunteers with an average age of 53.7±7,9.
Method: The levels of angiogenic factors in the serum samples taken before surgery, on day seven and week eight after surgery were determined by means of a protein array method by RayBiotech Company (USA), RayBio Human Angiogenesis Antibody Array I. The evaluation was carried out by ARES ARay (Baria, Czech Rep.). The resulting concentration of proteins was expressed as a relative value in comparison to controls.
Results: We found a significant pre-operative increase in IL-8 level in patients with stage III RCC (p=0.040) and stage IV RCC (p=0.017) compared to the control group. There was a significant post-operative decrease in IL-8 levels in all the groups of patients as early as day seven after surgery. Pre-operative serum levels of PDGF in patients with stages I and II RCC (p=0.017) and stage III RCC (p=0.029) were significantly increased compared to control group. On the contrary, in patients with stage IV RCC, it was lower compared to control group (p=0.032) and patients with stages I and II RCC (p<0.001) and stage III RCC (p=0.005). In patients with stage IV RCC the increase in PDGF reached statistical significance (p=0.003) on week eight.
Conclusion: The decrease in IL-8 in serum as early as day seven after surgery shows its significant production by the primary RCC cells. PDGF levels in serum did not change after the removal of primary RCC or they were gradually increasing in stage IV RCC, which proves other regulatory pathway than in IL-8. Supported by the grant from the IGA of the Czech Republic Ministry of Health: NR/8914-4.
Datum přednesení příspěvku: 12. 9. 2008
