Klin Onkol 2019; 32(4): 277-280. DOI: 10.14735/amko2019277.
Background: Long non-coding RNAs (lncRNA) have recently been the focus of attention of cancer researchers due to their diverse roles in the carcinogenesis process. These transcripts regulate critical steps in the normal cellular processes, so dysregulation of their expression participate in the pathogenesis of several cancers. P21-associated ncRNA DNA damage activated (PANDA) has a special situation in this regard due to its adjacency to the CDKN1A locus. It is involved in the regulation of DNA damage response as well as cell senescence and proliferation. Material and methods: In the current study, we assessed the expression of this lncRNA in bladder cancer tissue, adjacent non-cancerous tissues (ANCTs) and normal bladder samples by means of quantitative real time PCR method. Results: No significant difference has been detected in PANDA expression either between tumour tissue and ANCTs (expression ratio 1.75, P = 0.11) or between tumour tissue and normal tissues (expression ratio 2.72, P = 0.57). The expression level of this lncRNA was not associated with any of the demographic or clinical data of patients such as tumor grade or recurrence or cancer-associated risk factors such as cigarette smoking or opium addiction. Conclusion: Consequently, the current study implies that PANDA is not involved in the pathogenesis of bladder cancer. Assessment of expression of other lncRNAs would help in identification of biomarkers for this cancer.
