Klin Onkol 2008; 21(Suppl 1): 220-222.
Real-time PCR is a sensitive method for RNA analysis based on fl uorescence measurement. It is used in basic research, applied molecular medicine, and biotechnology. Real-time PCR assays are easy to perform and combine high sensitivity with reliability. The technology is evolving rapidly with the introduction of new reagents and instrumentation. It can be used for the confi rmation of
data acquired by microarray analysis of gene expression. We review basic principles of real-time PCR and describe its application in hematology and especially in multiple myeloma research.
