Klin Onkol 2005; 18(2): 59-63.
Summary:
Background: Chromosomal abnormalities, such as 13q deletion, are emerging as important prognostic factors in multiple myeloma. The availability of cytogenetic information has long been hampered by the low mitotic activity of the plasma cells. To obtain a direct assessment of the myeloma cells pathology in high purity cell population, we have used magneticsactivated cell sorting in combination with intephase fluorescence in situ hybridization.
Design and subject: The study included 40 patients with multiple myeloma.
Methods and results: We used chromosomal G-banding and interphase in situ hybridization on CD138+ and CD138cells after selection by magnetic-activated cell separation. Hybridization was performed to detect the 13q14 deletion in unselected bone marrow cells and, in parallel, in CD138+ enriched samples. The 13q14 deletion was found in 10 of 40 (25%) of bone marrow samples without cell selection and in 25 of 40 (62.5%) of samples with CD138+ enriched myeloma cells.
Conclusions: Our results confirm that immunomagnetic selection of CD138+ cells increases the probability of detection of the 13q14 deletion in bone marrow samples.
