Klin Onkol 2003; 16(5): 223-226.
Summary:
Backgrounds: Tumour invasiveness represents one of the key points in the metastatic process. The ability of invasion or a lack thereof result from interplay of several intermediate phenotypes, like cellular motility or an ability of proteolytic degradation of tissue barriers (basement membrane, extracellular matrix). One of the possibilities how to define a relationship between the invasiveness as a complex phenotype and the respective intermediate phenotypes is to analyze a defined tumour cell line series, with a relevant phenotypic polymorphism among individual cell lines. Design: We used a fibrosarcoma developed in the H2-K/v-jun transgenic mouse and by means of an extensive manipulation in tissue culture we generated a series of cell lines polymorphic in the expression of the invasive and motile phenotype.
Methods and Results: In establishing the series, three chief principles were exploited.
- Cellular heterogeneity within the original tumour sample was used to derive the founder cell lines JUN-1, -2, and –3.
- Spontaneous transformation upon continuous culture made it possible to distinguish sublines derived from the cell lines JUN-1 and JUN-2.
- Stable transfection of an expression vector for the cfos oncogene gave rise to an array of clones from individual JUN-1and JUN-2derived sublines.
Conclusions: The complete series of H2-K/v-jun fibrosarcomaderived cell lines enables to regard invasiveness and motility either as integral parts of a common phenotype, or independently of each other. As such, the series provides a convenient input into studies aimed at molecular characterization of invasive phenotype.
